LOCUS       sequence-76394-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplies Cre recombinase and Luciferase and IRES with flanked by 
            AttR2 and AttL3 sites for Gateway cloning recombination reaction. 
            (This corresponds to a module 3 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-iCreT2ALuc-L3 #BGV208
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..94)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggggccaggg ttctcctcca
       61 cgtcgccgca ggtcagcagg ctgcctctgc cctcgccgct gccgccggca tcgccatctt
      121 ccagcaggcg caccattgcc cctgtttcac tatccaggtt acggatatag ttcatgacaa
      181 tatttacatt ggtccagcca ccagcttgca tgatctccgg tattgaaact ccagcgcggg
      241 ccatatctcg cgcggctccg acacgggcac tgtgtccaga ccaggccagg tatctctgac
      301 cagagtcatc cttagcgccg taaatcaatc gatgagttgc ttcaaaaatc ccttccaggg
      361 cgcgagttga tagctggctg gtggcagatg gcgcggcaac accatttttt ctgacccggc
      421 aaaacaggta gttattcgga tcatcagcta caccagagac ggaaatccat cgctcgacca
      481 gtttagttac ccccaggcta agtgccttct ctacacctgc ggtgctaacc agcgttttcg
      541 ttctgccaat atggattaac attctcccac cgtcagtacg tgagatatct ttaaccctga
      601 tcctggcaat ttcggctata cgtaacaggg tgttataatc aatccccaga aatgccagat
      661 tacgtatatc ctggcagcga tcgctatttt ccatgagtga acgaacctgg tcgaaatcag
      721 tgcgttcgaa cgctagagcc tgttttgcac gttcaccggc atcaacgttt tcttttcgga
      781 tccgccgcat aaccagtgaa aca
//
