LOCUS       sequence-76406-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G1-Ph
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(298..487)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(517..546)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(563..582)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(564..582)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggatctt
      301 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg
      361 tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac
      421 tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct tttcggttag
      481 agcggatctt agctagccgc ggtaccaagc ttactcgagg tcttcttcgg aaatcaactt
      541 ctgttccatg tcgacgcccg ggccctatag tgagtcgtat tacggatcct tgttttatat
      601 ttgttgtaaa aagtagataa ttacttcctt gatgatctgt aaaaaagaga aaaagaaagc
      661 atctaagaac ttgaaaaact acgaattaga aaagaccaaa tatgtatttc ttgcattgac
      721 caatttatgc aagtttatat atatgtaaat gtaagtttca cgaggttcta ctaaactaaa
      781 ccaccccc
//
