LOCUS       sequence-76414-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH78141
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..211
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(491..509)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        577..681
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      241 gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcatac tgtataagag
      301 ctcattttta caacaattac caacaacaac aaacaacaaa caacattaca attacattta
      361 caattatcga taacatgggg aagcaaatgg ccgccctgtg tggctttctc ctcgtggcgt
      421 tgctctggct cacgcccgac gtcgcgtcag caatgtgaga ccacgaagtg gctcttcagt
      481 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      541 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      601 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      661 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
      721 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg
      781 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
      841 tgttatggag cagcaacgat gttacgcagc agggca
//
