LOCUS       sequence-76424-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45131
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(681..699)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        767..871
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc
       61 actctgtggt ctcaggagtt gttgccgaaa acattcaaca aaatcaatca aaggtatcag
      121 ttaatatcat tgtcgacggt tttatattat taacttatga tcgtaagaac ttcgtatcac
      181 tttttgtttc atctaactgc attgttttac ataacattga tatatatttg tttggtaagt
      241 gtttctagtt tcacttaaaa actttgtagt ttggcgttcg aaacaaactt gataagaaaa
      301 aaaagaaaat attattgaga gcatgaagtg gattggaatt aaagaaaaca aggaaaaaga
      361 aaaaaataga ttggaataat ttcaaatatt tctttccaat aattcaagca tttatctttg
      421 aaaatttccc caaagtttct ttgtgtacaa atgacaagtg ttttcgttgc agctaaagaa
      481 tctagcaaaa gcttatggac tagagagtga cacataggac aagataacga caatcccaaa
      541 atctcataag ccaatcacta aacagataca gatacttcac agataacaaa ccttactctt
      601 aacattgcta taaagtggac gtatacccca accattttca atacttgaga ccacgaagtg
      661 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      721 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      781 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      841 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
      901 gaccgaacgc agcggtggta acggcgca
//
