LOCUS       sequence-76425-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL12006
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(634..652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        720..824
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc
       61 actctgtggt ctcaggagcc agaaggtaat tatccaagat gtagcatcaa gaatccaatg
      121 tttacgggaa aaactatgga agtattatgt aagctcagca agaagcagat caatatgcgg
      181 cacatatgca acctatgttc aaaaatgaag aatgtacaga tacaagatcc tatactgcca
      241 gaatacgaag aagaatacgt agaaattgaa aaagaagaac caggcgaaga aaagaatctt
      301 gatgacgtaa gcactgacga caacaatgaa aagaagaaga taaggtcggt gattgtgaaa
      361 gagacataga ggacacatgt aaggtggaaa atgtaagggc ggaaagtaac cttatcacaa
      421 aggaatctta tcccccacta cttatccttt tatatttttc cgtgtcattt ttgcccttga
      481 gttttcctat ataaggaacc aagttcggca tttgtgaaaa caagaaaaaa tttggtgtaa
      541 gctattttct ttgaagtact gaggatacaa cttcagagaa atttgtaagt ttgtaatgtg
      601 agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg
      661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact
      841 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
      901 gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      961 ttacgccg
//
