LOCUS       sequence-76435-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Please note- this plasmid is currently not distributed in the MoClo 
            Plant Parts Kit (# 1000000047).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50015
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     regulatory      561..570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca ctgtggtctc
      301 attcgatggt gagcaaggga gaggaggata acatggcctc tctcccagct acacatgagc
      361 ttcacatctt tggatccatc aacggtgtgg actttgacat ggtgggtcag ggaaccggaa
      421 atccaaatga tggatatgag gagcttaacc ttaagtccac caagggtgac ctccagttct
      481 ccccatggat tcttgtccct catatcggat atggattcca tcagtacctt ccttaccctg
      541 acggtatgtc tcctttccag gccgccatgg ttgatggatc cggataccaa gtccatagaa
      601 caatgcagtt tgaagatggt gcctccctta ctgttaacta cagatacacc tacgagggaa
      661 gccacatcaa aggagaggcc caggtgaagg gaactggttt ccctgctgac ggtcctgtga
      721 tgaccaactc tcttaccgct gctgactggt gcaggtctaa gaaaacttac cctaacgaca
      781 aaaccatcat cagtaccttt aagtggagtt acaccactgg aaatggtaag agatacagaa
      841 gcactgctag aaccacctac acctttgcca agccaatggc tgctaactat ct
//
