LOCUS       sequence-76438-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a GFP-dependent transcription factor component in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pCAG-GBP1-10gly-Gal4DBD
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             128..469
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYSNVNVGFEYW
                     GQGTQVTVSS"
ORIGIN
        1 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
       61 tttggcaaag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtgccaccat
      121 ggccgacgtg cagctcgtgg aatccggagg agcactggtc cagcctgggg gatcactgag
      181 actgtcttgt gccgcttctg gctttcctgt gaatcggtac tctatgagat ggtacaggca
      241 ggcacctgga aaagagcgag aatgggtcgc cggaatgtca tctgctggcg ataggagttc
      301 ctacgaggat agcgtgaaag gacggttcac cattagtagg gacgatgcta gaaacaccgt
      361 gtacctccag atgaactccc tgaaacctga ggacactgct gtgtactact ccaacgtcaa
      421 tgtgggcttt gaatactggg gacagggcac acaagtgact gttagttctg ctagcggagg
      481 aggtggtgga ggtggaggtg gaggtactag tatgaagcta ctgtcttcta tcgaacaagc
      541 atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa
      601 gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac
      661 tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact
      721 gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa
      781 agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag
      841 attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
//
