LOCUS       sequence-76440-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_AACA
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(526..543)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(526..542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(535..557)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      606..692
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagacgt ctcgatagcc accacttggt ccggggtcag gccatggtcc tggcacagca
      121 ccggcaacag ccgctgcacc gtttcgagcg cttgcttgcc gccaatgttg ctggcgatag
      181 ccaccacttg gtccggggtc aggccatggt cctggcacag caccggcaac agccgctgca
      241 ccgtttcgag cgcttgcttg ccgccatcgt ggctggcgat agccaccact tggtccggag
      301 tcaggccatg gtcctggcac agcaccggca acagccgctg caccgtttcg agcgcttgct
      361 tgccgccaat gttgctggcg atagccacca cttggtccgg ggtcaggcca tggtcctggc
      421 acagcaccgg caacagccgc tgcaccgttt cgagcgcttg cttgccgcca atgttgctgg
      481 cgatagccac cacttggtcc ggggtcaggc catgagacgc ttaagactgg ccgtcgtttt
      541 acaacgtcgt gactgggaaa acatccatgc tagcgttaac gcgagagtag ggaactgcca
      601 ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt
      661 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aa
//
