LOCUS       sequence-76446-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL70004
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..211
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        366..545
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    576..630
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     complement(686..705)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      241 gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcagga gcggagaatt
      301 aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac
      361 tgacagaacc gcaacgttga aggagccact gagccgcggg tttctggagt ttaatgagct
      421 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
      481 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc
      541 aattagagtc tcatattcac tctcctattt ttacaacaat taccaacaac aacaaacaac
      601 aaacaacatt acaattacat ttacaattac catggttgaa caagatggat tgcacgcagg
      661 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
      721 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
      781 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
      841 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
      901 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcac
//
