LOCUS       sequence-76447-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH77911
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..211
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(737..755)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      241 gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcagct tgaattaaca
      301 gaggtggatg gacagacccg ttcttacacc ggactgggcg cgggatagga tattcagatt
      361 gggatgggat tgagcttaaa gccggcgctg acaccatgct caaggtaggc aatgtcctca
      421 gcgtcgagcc cggcatctat gtcgagggca ttggtggagc gcgcttcggg gataccgtgc
      481 ttgtaactga gagcggatat gaggccctca ctccgcttga tcttggcaaa gatatttgac
      541 gcatttatta gtatgtgtta attttcattt gcagtgcagt attttctatt cgatctttat
      601 gtaattcgtt acaattaata aatattcaaa tcagattatt gactgtcatt tgtatcaaat
      661 cgtgtttaat ggatattttt attataatat tgatgatcgc ttgagaccac gaagtggctc
      721 ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      781 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      841 ttatttttct aaatacattc aaatatgtat cc
//
