LOCUS       sequence-76449-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH42211
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..211
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        368..547
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(607..625)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        693..797
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      241 gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcagga gagcggagaa
      301 ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt tacgtttgga
      361 actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg ggtttctgga gtttaatgag
      421 ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag gtcactatca
      481 gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc ccctcggtat
      541 ccaattagag tctcatattc actctcatac ttgagaccac gaagtggctc ttcagtggac
      601 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      661 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      781 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      841 gtggtaacgg cgca
//
