LOCUS       sequence-76484-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50006
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(143..161)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc
       61 actgtggtct cattcgatgg aggacgccaa aaacataaag aaaggcccgg cgccattcta
      121 tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
      181 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga
      241 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
      301 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
      361 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
      421 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa
      481 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa
      541 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
      601 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat
      661 aatgaattcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc
      721 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
      781 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
      841 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaa
//
