LOCUS       sequence-76491-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH85281
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        308..688
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(728..746)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        814..918
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
       61 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      121 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      181 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      241 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc
      301 aggagatttt tcaaatcagt gcgcaagacg tgacgtaagt atccgagtca gtttttattt
      361 ttctactaat ttggtcgttt atttcggcgt gtaggacatg gcaaccgggc ctgaatttcg
      421 cgggtattct gtttctattc caactttttc ttgatccgca gccattaacg acttttgaat
      481 agatacgctg acacgccaag cctcgctagt caaaagtgta ccaaacaacg ctttacagca
      541 agaacggaat gcgcgtgacg ctcgcggtga cgccatttcg ccttttcaga aatggataaa
      601 tagccttgct tcctattata tcttcccaaa ttaccaatac attacactag catctgaatt
      661 tcataaccaa tctcgataca ccaaatcgaa tgtgagacca cgaagtggct cttcagtgga
      721 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      781 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      901 tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaa
//
