LOCUS       sequence-76493-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44233
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(430..448)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        516..620
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
       61 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      121 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      181 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      241 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc
      301 atactgttta tttacaagag cgtacggttc aacccctgcc tcccctgtaa aactccctag
      361 acttaaatct tttctttcta gtatcttttc aatgtgagac cacgaagtgg ctcttcagtg
      421 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      481 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      541 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      601 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      661 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt
      721 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
      781 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
      841 catga
//
