LOCUS       sequence-76499-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses UVR8 fused to GFP with a prenylation motif for membrane 
            targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Chen D, Gibson ES, Kennedy MJ
  TITLE     A light-triggered protein secretion system.
  JOURNAL   J Cell Biol. 2013 May 13;201(4):631-40. doi: 10.1083/jcb.201210119.
  PUBMED    23671313
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-05-13- 13"; volume: "201"; 
            issue: "4"; pages: "631-40"
COMMENT     Sequence Label: UVR8-GFPCaaX
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(183..893,894..896,897..899))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(183..893,894..896,897..899))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(225..246)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     574..593
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     833..854
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      896..905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
       61 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctag agtcgcggcc
      121 gctttacttg tacactacat aattacacac tttgtctttg acttcttttt cttcttttta
      181 cccttataca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      241 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
      301 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      361 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
      421 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
      481 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      541 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      601 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
      661 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
      721 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      781 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      841 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
      901 gtggcgaccg gtggatcccg ggcccgcggt accgtc
//
