LOCUS       sequence-76503-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of SYFP2-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQYD
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             299..364
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(436..453)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(436..452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(445..467)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(661..680)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     780..802
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(840..858)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      181 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      241 ttcttgtaca aagtggtgat atcgctagct agaggtaccg agctccaatt gggatccgga
      301 ctacaaggac cacgacggcg actataagga ccacgacatc gactacaagg atgacgacga
      361 caagtgagga tccggtgaag tgacccgcaa aaaaaaaggc caggggatcg ctcccccggc
      421 cttttttttg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      481 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      541 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
      601 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      661 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
      721 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      781 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
      841 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagcaccc
      901 tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc
      961 accgcgagtc cctgctcgaa cgctgcgtcc ggaccggc
//
