LOCUS       sequence-76505-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of SYFP2-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQYD
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..81
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    124..151
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atgcggaagc ttcctctact agttacaaac
       61 agaccagatt gtctgtttgt taaccggcgc agttagcggc ttcgcggcga tccgtcaacc
      121 atgtacaaac agaccagatt gtctgtttgt tccggtacat tgacatatgt atccgctcat
      181 gatacaataa ccctgatgct agcagggaga gaccccgaat ggtcatcatg tcgccgaagc
      241 gccgcaccca ggccgagcgc gcgatggaaa cccagggcaa gctgatcgcc gccgccctgg
      301 gcgtcctgcg cgagaagggc tatgccggct tccgcatcgc cgacgtcccg ggcgccgccg
      361 gcgtcagccg cggcgcccag tcgcaccact tcccgaccaa gctggaactg ctgctggcca
      421 ccttcgagtg gctgtacgag cagatcaccg agcgcagccg cgcccgcctg gccaagctga
      481 agcccgagga cgacgtcatc cagcagatgc tggacgacgc cgccgagttc ttcctggacg
      541 acgacttctc gatctcgctg gacctgatcg tggccgccga ccgcgacccg gcgctgcgcg
      601 agggcatcca gcgcaccgtc gagcgcaacc gcttcgtcgt cgaggatatg tggctgggcg
      661 tgctggtgtc gcgcggcctg agccgcgacg acgcggagga catcctgtgg ctgatcttca
      721 actcggtccg cggcctggcg gtccgctcgc tgtggcagaa ggacaaggag cgcttcgagc
      781 gcgtccgcaa ctcgac
//
