LOCUS       sequence-76510-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of 3xFLAG-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQFD
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..137)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    179..303
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(445..464)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gaattcaaaa aaaaggccgg gggagcgatc ccctggcctt tttttttgcg ggtcacttca
       61 ccggatcctc acttgtcgtc gtcatccttg tagtcgatgt cgtggtcctt atagtcgccg
      121 tcgtggtcct tgtagtccgg atcccaattg gagctcggta cctctagcta gcgatatcac
      181 cactttgtac aagaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      241 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      301 atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
      361 tcaggttaat ggcgttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc
      421 gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc
      481 gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc
      541 cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa
      601 cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc
      661 tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt
      721 cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg
      781 tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct
      841 gtcaactgtc actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta
      901 tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat
      961 ctggctttta gtaagccgga tccacgcg
//
