LOCUS       sequence-76511-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71431
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(596..614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        682..786
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc
       61 actctgtggt ctcagcttac cgcactgtgt gtggtttctc aagaccaaga cagctaaagc
      121 ctaaagtcag agatctaata tgtgtattgt tattcatgac accacagctg ccacttttgg
      181 tgttatgatc tgtttgtaga agtaggaatt cttttttttc tacttaataa tagcttaaag
      241 agctgtgcaa tttggtctgt attttttgtg tattttgcac tcattatttg tgaacagttt
      301 gagaactatt tattttctaa gatttgtgca cgtatgaacc acttttcatc tatataccac
      361 catgtttatt ctgcatctat gggattgagt ttgaatattc gttgatcaac aaagttatat
      421 ttggtggata ctacttgaag gtgcatatac tttgtgctca tatatttagt tgatattctg
      481 gattttgagc tggacaaatt gatcaaggta gtctaatctg gtctggttac taataaaact
      541 caagagatca ctgtctcgct tgagaccacg aagtggctct tcagtggacg aaagggcctc
      601 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      661 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      721 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      781 aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tg
//
