LOCUS       sequence-76514-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH42760
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(540..558)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        626..730
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
       61 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      121 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      181 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      241 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc
      301 aggaggtcaa aggagtgtca gttaattaaa gagtgtcggg gccgacactc ttaatggaag
      361 taaaagtgtg ggttttgctg caccgacact cttaatttaa gagtgtcggg gtcccgatga
      421 aaccgacgct tttaatttaa gagtgtgggt ttttccacac cgacactctt atgaatgtta
      481 ccctaaattc cccaatccta tacttgagac cacgaagtgg ctcttcagtg gacgaaaggg
      541 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      721 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
      781 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
      841 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatgga
//
