LOCUS       sequence-76521-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cumate-inducible expression of SYFP2-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQY
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..74
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    117..144
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             221..832
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
ORIGIN
        1 acagctcctc gcccttgctc accatgcgga agcttcctct actagttaca aacagaccag
       61 attgtctgtt tgttaaccgg cgcagttagc ggcttcgcgg cgatccgtca accatgtaca
      121 aacagaccag attgtctgtt tgttccggta cattgacata tgtatccgct catgatacaa
      181 taaccctgat gctagcaggg agagaccccg aatggtcatc atgtcgccga agcgccgcac
      241 ccaggccgag cgcgcgatgg aaacccaggg caagctgatc gccgccgccc tgggcgtcct
      301 gcgcgagaag ggctatgccg gcttccgcat cgccgacgtc ccgggcgccg ccggcgtcag
      361 ccgcggcgcc cagtcgcacc acttcccgac caagctggaa ctgctgctgg ccaccttcga
      421 gtggctgtac gagcagatca ccgagcgcag ccgcgcccgc ctggccaagc tgaagcccga
      481 ggacgacgtc atccagcaga tgctggacga cgccgccgag ttcttcctgg acgacgactt
      541 ctcgatctcg ctggacctga tcgtggccgc cgaccgcgac ccggcgctgc gcgagggcat
      601 ccagcgcacc gtcgagcgca accgcttcgt cgtcgaggat atgtggctgg gcgtgctggt
      661 gtcgcgcggc ctgagccgcg acgacgcgga ggacatcctg tggctgatct tcaactcggt
      721 ccgcggcctg gcggtccgct cgctgtggca gaaggacaag gagcgcttcg agcgcgtccg
      781 caactcgacc ctggaaatcg cccgcgagcg ctacgccaag ttcaagcgct gaacatgtga
      841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      961 ccgacaggac tataaagata ccaggcgt
//
