LOCUS       sequence-76523-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for the Golden Gate TALEN kit, for in vitro 
            synthesis of TALEN mRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian 
            M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC
  TITLE     Efficient TALEN-mediated gene knockout in livestock.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 1.
  PUBMED    23027955
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Oct 1."
COMMENT     Sequence Label: RCIscript-GoldyTALEN
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        131..235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             420..798
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(454..473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
       61 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
      121 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      181 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      241 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      301 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      361 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      421 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      481 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      541 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      601 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
      661 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      721 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
      781 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
      841 gcaacaatta atagactgga tggaggcgg
//
