LOCUS       sequence-76538-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH41414
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    337..511
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(550..568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        636..740
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tctgtggtct
      301 cagcttctct agctagagtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta
      361 gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag
      421 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
      481 aaaaccaaaa tccagtacta aaatccagat cgcttgagac cacgaagtgg ctcttcagtg
      541 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      601 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      661 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      721 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      781 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt
      841 acagtctatg cctc
//
