LOCUS       sequence-76546-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44157
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(824..842)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
       61 attcattaat cactctgtgg tctcaggagg cttaagtggg ccgtaagtgc aattgcgttg
      121 tgctccaaaa ggaacggtgc cgtgggttcc tcttgttcat cagaaatata ttaattagcc
      181 gtaaaacctg aaaattcaca agcatttgga ttgttttcta atttaatatc cattatgtga
      241 ctaaaagttc tagtgatcgt acatactaca tagaaaataa taacacaaaa tactagttta
      301 catttcccaa ttaaaaacca ttttgaatga actctgtctg atttaattat acttttaaaa
      361 tgtgggatga attcaaagat tatacttata ttcttattat ttaagattat caagtggaaa
      421 aataaaaata tgaatgtgtt aatataaggt aatagaaatt taatcatttt tttaatctat
      481 atgtaaaaag tatttaaccg atatctacaa tttgacgcct cccaattgaa aggagccaaa
      541 agcaaccgat caagtgcaga ccagtagcca tacacattca ctcctaccct tacatgagaa
      601 agataagatt atggagtttt ctgccacgtg atcttatcct agtggtccaa atcgataagg
      661 gtgtcaacac ctttccttaa tcctgtggca attaacgacg ttatcatgaa ttatggcccc
      721 tttgatcatt agggctagtt gcctctagcg gttcccacta tataaagatg acaaaaccaa
      781 cagatacttg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta
      841 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      901 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tac
//
