LOCUS       sequence-76554-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Dantuma NP, Lindsten K, Glas R, Jellne M, Masucci MG
  TITLE     Short-lived green fluorescent proteins for quantifying 
            ubiquitin/proteasome-dependent proteolysis in living cells.
  JOURNAL   Nat Biotechnol. 2000 May . 18(5):538-43.
  PUBMED    10802622
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2000 May . 18(5):538-43."
COMMENT     Sequence Label: Ub-R-GFP
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(62..289)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     promoter        complement(359..562)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(388..408)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        563..866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ctgtcgacca agcttcccgc
       61 gcccacctct gagacggagt accaggtgca gggtggactc tttctggatg ttgtagtcag
      121 acagggtgcg cccatcttcc agctgttttc cagcaaagat cagcctctgc tggtcaggag
      181 ggatgccttc cttatcttgg atctttgcct tgacattctc aatggtgtca ctgggctcaa
      241 cctcgagggt gatggtctta ccagtcagag tcttcacgaa gatctgcatg gtgaattcga
      301 agcttgagct cgagatctga gtccggtagc gctagcggat ctgacggttc actaaaccag
      361 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      421 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      481 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      541 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      601 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      661 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
      721 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      781 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      841 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
      901 ccgtaattga ttactatta
//
