LOCUS       sequence-76558-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a GFP-dependent transcription factor component in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pCAG-GBP7-p65AD
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(176..547)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc
       61 ccataatttt tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc
      121 acaccagcca ccaccttctg ataggcagcc tgcacctgag gagtgcggcc gcttaggagc
      181 tgatctgact cagcagggct gagaagtcca tgtccgcaat ggaggagaag tcttcatctc
      241 ctgaaaggag gccattgggg agccccgggg cccccagtgg agcaggagct gggtcggggg
      301 gcctctgggc ccctgtcact aggcgagtta tagcctcagg gtactccatc agcatgggct
      361 cagttgtgtg gggggccaca ggtatgccct ggttcagcag ctgctgaaac tcggagttgt
      421 cgacggatgc caggtctgtg aacacagctg ggtctgtgct gttgccaagc aaggccccca
      481 ggtcttcatc atcaaactgc agctgcagca gggcctctga cagcgttcct tccccagcct
      541 gggtgggctt gggggcaggt ggggccacag cctgaggagg gcctggggct aggactggga
      601 caggggctgg ggcctgggcc agagctgata ccatggctgg agcaggggca ggggctggag
      661 cctggggcag gacttgggga ggggccgggg ccaaggccga ggcctggctg atctgcccag
      721 aaggcaa
//
