LOCUS       sequence-76563-e         525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multi-purpose expression of a GFP-dependent transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pCS2+ Gal4-GBP2-IRES-GBP7-p65
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..41
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     23..40
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             79..519
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct acttgttctt
       61 tttgcaggat ccgccaccat gaagctactg tcttctatcg aacaagcatg cgatatttgc
      121 cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac
      181 aactgggagt gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg
      241 acagaagtgg aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga
      301 gaagaccttg acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca
      361 ggattatttg tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg
      421 gagactgata tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa
      481 gagagtagta acaaaggtca aagacagttg actgtatcgg ctagc
//
