LOCUS       sequence-76571-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an attenuated T7 RNAP under an isopropyl 
            beta-d-1-thiogalactopyranoside (IPTG) inducible control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pN565
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..41)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     misc_feature    77..97
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 ggaagtttta gtcaaaagcc tccggtcgga ggcttttgac tttctgctaa tctaactaac
       61 taacccttag tgactcctgc agcggccgct actagtatta cgcgaacgcg aagtccgact
      121 ctaagatgtc acggaggttc aagttacctt tagccggaag tgctggcatt ttgtccaatt
      181 gagactcgtg caactggtca gcgaactggt cgtagaaatc agccagtaca tcacaagact
      241 catatgtgtc aaccatagtt tcgcgcactg ctttgaacag gttcgcagcg tcagccggaa
      301 tcgtaccgaa ggagtcgtga atcagtgcaa aagattcgat tccgtacttc tcgtgtgccc
      361 acactacagt cttacgaagg tggctaccgt cttggctgtg tacaaagtta ggagcgatac
      421 cagactcctg tttgtgtgca tcaatctcgc tatctttgtt ggtgttaatg gtaggctgta
      481 agcggaactg accgaggaac atcaggttca agcgcgtctg aataggcttc ttgtattcct
      541 gccacacagg gaaaccatca ggagttaccc aatgcacagc gcaacgcttg cgaagaatct
      601 ctccagtctt cttatctttg acctcagcag ccagcagctt agcagcagac ttaagccagt
      661 tcattgcttc aaccgcagct accaccgtca cgctcacaga ttcccaaatc agcttagcca
      721 tgtatccagc agcctgattc ggctgagtga acatcagacc cttgccggaa tc
//
