LOCUS       sequence-76597-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human SRP14A5 and SRP9 proteins as a fusion protein in 
            mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  TITLE     Strub Lab 14-9VN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p14-9VNA5
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..46
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             318..326
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      773..782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(824..845)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccgtcagaat tgatctacca tggactacaa agacgatgac gacaagcttg cggccgcgaa
       61 ttctatggtg ttgttggaga gcgagcagtt cctgacggag ctgaccagac ttttccagaa
      121 gtgtcggacg tcgggcagcg tctatatcac cttgaagaag tatgacggtc gaaccaaacc
      181 cattccaaag aaaggtactg tggagggctt tgagcccgca gacaacaagt gtctgttaag
      241 agctaccgat gggaagaaga agatcagcac tgtggtgagc tccaaggaag tgaataagtt
      301 tcagatggct tattcaaacc tccttagagc taacatggat gggttgaagg cagcagcagc
      361 agcaaacaaa actaagaaga ccaaagcagc agcaacagca gcagcagcag cacctgccgc
      421 agcagcaaca gcagcaacaa cagcagcaac aacagcagca acagcagcac agtcccccgg
      481 gggaatgccg cagtaccaga cctgggagga gttcagccgc gctgccgaga agctttacct
      541 cgctgaccct atgaaggcac gtgtggttct caaatatagg cattctgatg ggaacttgtg
      601 tgttaaagca acagatgatt tagtttgttt ggtgtataaa acagaccaag ctcaagatgt
      661 aaagaagatt gagaaattcc acagtcaact aatgcgactt atggtagcca aggaagcccg
      721 caatgttacc atggaaactg agggggtacc agtcgactct agaagatcca tcgccaccat
      781 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      841 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      901 caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccc
//
