LOCUS       sequence-76598-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human SRP14 and SRP9-3A proteins as a fusion protein in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     Strub Lab 14-9VN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p14-9VN9-3A
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..62
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             334..342
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      789..798
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(840..861)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agagctcgtt tagtgaccgt cagaattgat ctaccatgga ctacaaagac gatgacgaca
       61 agcttgcggc cgcgaattct atggtgttgt tggagagcga gcagttcctg acggagctga
      121 ccagactttt ccagaagtgt cggacgtcgg gcagcgtcta tatcaccttg aagaagtatg
      181 acggtcgaac caaacccatt ccaaagaaag gtactgtgga gggctttgag cccgcagaca
      241 acaagtgtct gttaagagct accgatggga agaagaagat cagcactgtg gtgagctcca
      301 aggaagtgaa taagtttcag atggcttatt caaacctcct tagagctaac atggatgggt
      361 tgaagaagag agacaaaaag aacaaaacta agaagaccaa agcagcagca acagcagcag
      421 cagcagcacc tgccgcagca gcaacagcag caacaacagc agcaacaaca gcagcaacag
      481 cagcacagtc ccccggggga atgccgcagt accagacctg ggaggagttc agccgcgctg
      541 ccgagaagct ttacctcgct gaccctatga aggcacgtgt ggttctcaaa tataggcatt
      601 ctgatgggaa cttgtgtgtt aaagcaacag atgatttagt ttgtttggtg tataaaacag
      661 accaagctca agatgtagca gcaattgagg ccttccacag tcaactaatg cgacttatgg
      721 tagccaagga agcccgcaat gttaccatgg aaactgaggg ggtaccagtc gactctagaa
      781 gatccatcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      841 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      901 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
      961 tgccctgg
//
