LOCUS       sequence-76608-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44022
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(628..646)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        714..818
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg
       61 tggtctcaaa tggaacgagc tatacaagga aacgatgcta gggaacaagc ttatggtgaa
      121 cgttggaatg gaggatcagg aagttccact tctcccttca aacttcctga cgaaagtccg
      181 agttggactg agtggcggct acataacgat gagactattt cgaatcaaga taatcccctt
      241 ggtttcaagg aaagctgggg tttcgggaaa gttgtattta agagatatct cagatacgac
      301 gggacggaaa cttcactgca cagagtcctt ggatcttgga cgggagattc ggttaactat
      361 gcagcatctc gatttctcgg tttcgaccag atcggatgta cctatagtat tcggtttcga
      421 ggagttagtg tcaccatttc tggagggtcg cgaactcttc agcatctcag tgaaatggca
      481 attcggtcta agcaagaact gctacagctt accccagtca aagtggaaag tgatgtatca
      541 agaggatgcc ctgaaggtgt tgaaaccttc gaagaagaaa gcgagtaagc tttgagacca
      601 cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      661 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca
      841 gaaccttgac cgaacgcagc ggtggtaacg gcgca
//
