LOCUS       sequence-76616-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL30005
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     189..206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(285..308)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(404..422)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        490..594
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
       61 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      121 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      181 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      241 tccccgcgcg ttggccgatt cattaatcac tcgtggtctc acattttatc gtcatcgtcc
      301 ttataatcag ctgctgcagc gtcgaggtca tggtccttat agtctccgtc atggtcctta
      361 taatccatgg tgagaccaca gtggctcttc agtggacgaa agggcctcgt gatacgccta
      421 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc
      601 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
      661 ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc
      721 agcaagcgcg ttacgccgtg gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
      781 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
      841 gtatcgactc aactatca
//
