LOCUS       sequence-76621-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50016
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     intron          683..871
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tgtggtctca
      301 ttcgagagga tctggatctg agtctgatga gtctggactt cctgctatgg aaatcgagtg
      361 tagaatcact ggaaccctta acggtgttga gttcgagctt gttggaggtg gtgagggaac
      421 tcctgagcag ggaagaatga ctaacaagat gaagtctacc aagggtgctc ttaccttctc
      481 tccatacctt ctttctcacg ttatgggata cggattctac cacttcggaa cttacccatc
      541 tggatacgag aaccctttcc ttcatgctat caacaacggt ggatacacca acactaggat
      601 cgagaagtac gaggatggtg gtgttcttca cgttagcttc tcttacagat acgaggctgg
      661 aagagtgatc ggagatttca aggtaagttt ctgcttctac ctttgatata tatataataa
      721 ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta
      781 gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc
      841 taatatatga ccaaaatttg ttgatgtgca ggttatggga actggattcc ctgaggattc
      901 tgttatcttc accgacaaga tcatc
//
