LOCUS       sequence-76626-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL80012
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    338..359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(374..379,380..397,398..404)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    412..428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     417..439
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     436..452
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     436..452
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      301 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      361 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      421 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt gcatgcctgc
      481 aggtcgactc tagaggatcc cctttgaaga caattacgag gatgcacatg tgaccgaggg
      541 attgtcttcg tggaaacttg ggacttcaga ttccacccct tgcaagcgtg gaaagccttc
      601 cgaccaaggg aagtgtcgga tgtagaggaa atggaaagtt tgttctcaga tggggacttg
      661 cttgattgct tcacaagaat gccagcttat gcggtaaacg cagaggaaga tttagctgca
      721 atcaggaaaa cgcccgagat ggatgtcggt caagaagtta aagaacctgc aggagacaga
      781 aatcaatact caaaccctgc agaaactttc ctcaacaagc tccacaggaa acacagtagg
      841 gaggtgaaac accaggccgc
//
