LOCUS       sequence-76628-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH41421
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      315..567
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(609..627)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        695..799
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tctgtggtct
      301 cagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
      361 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
      421 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
      481 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
      541 gcgcggtgtc atctatgtta ctagatcgac gcttgagacc acgaagtggc tcttcagtgg
      601 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      661 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      781 atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      841 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttt
//
