LOCUS       sequence-76632-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50011
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(165..269)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     337..355
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
       61 ctgtacccca aaaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
      121 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatactctt cctttttcaa
      181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      301 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      361 cgtccactga agagccacgt ggtctcaaag ctcacctcct agcgttgtca ggcaatccag
      421 agtgtgccac tgcatgctcg tacaacttaa ccttattgta gtccttgtcg tgagacaaaa
      481 tctcgatgca gtggtcaacg aagtggtatc caggcaactt cactcccttc tccttagcct
      541 tgtatgtagt cctgaagtcg cacctgtagt gagcgttacc ctcaagcaac aatgccatgt
      601 ggatgtcacc agtcaacact ccgtccctca cgtacatctt ctctgtagaa ggctcccatt
      661 tcaatgtttt tttctgcatc acaggtccgt ttgcagggaa gttagttccg tagaacctca
      721 ccttgttgta aaatgtgtct ccctccattg tgatgtcgtt ccttgcgatg cagatacctc
      781 cgtcctcgaa agtcaatgat ctctcccaag agtaaccctt tgggaatgac tgcttgaagt
      841 aatcttgaat gttatcaggg tacttagcga aaaccctgtt accgta
//
