LOCUS       sequence-76634-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bmal fragment cloned with Venus tag used for mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: HLH Bmal Pas 1-465-VenC L150E
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(130..381)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(172..193)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      384..393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
       61 tacaaatgtg gtatggctga ttatgatcag ttatctagat ccggtggatc ccgagggcag
      121 cattccggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      181 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actggtagct
      241 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      301 gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg cggatcttga agttggcctt
      361 gatgccgttc ttctgcttgt ccaccatggt ggcgaccggg ctgtccatgc tgtggggggg
      421 tgctgttagc tgcgggaagg ttgggtcccc gccttccagg acattggcta aaacaacagt
      481 gttggttgag acaatgtatt caacttcctt ggtccacggg ttcatgaaac tgaaccatcg
      541 acttcgtagc gtgataaaag aaccatcttt gatcttaaac ttatagcaat tagttgtgat
      601 cttttctctt gtctgtaaaa cttgcctgtg acattctgcg aggtgtccta tgtcgtcttg
      661 atgaaaatac tcataacatg atgtacctag aagttcctgt ggtagatacg ccaaaatagc
      721 tgtcgccctc tgatctacaa aaacaaattt cccatctatt gcgtgtcgag aaacgtactc
      781 catagatttc acccgtattt ccccgttcgc tggttgtgga accatgtgcg agtgcaggcg
      841 cccgattgca acgaggcagc tgaggttgca gccctcgttg tctggctcat tgtcttcgtc
      901 cagccccatc ttcgtgggtg g
//
