LOCUS       sequence-76636-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL70002
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(165..269)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     337..355
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    418..592
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
       61 ctgtacccca aaaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
      121 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatactctt cctttttcaa
      181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      301 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      361 cgtccactga agagccactt cgtggtctca agcggaattc ggatatcggt cgacggggat
      421 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt
      481 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat
      541 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aagcttattt
      601 ttctgcacta cgcagggata tttcaccgcc catccagggt tttattattc catcctgctc
      661 aagtaataaa gccccctgtt tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc
      721 aatgataagt ttcactgggc gattaataaa attatccagc ttttcccagc gcgacagata
      781 aggtgccaat ccttcttgtt cgaagagttc caacgcagca cgtaattcac gtattagcat
      841 ggccgccaac gtattacgat cgagattgat ccccgcttcc tgcagcgtga tccacccctg
      901 attaacgaca ctctcttcaa cacggcgcat
//
