LOCUS       sequence-76639-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45089
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(165..269)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     337..355
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        complement(398..743)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(435..455)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
       61 ctgtacccca aaaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
      121 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatactctt cctttttcaa
      181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      301 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      361 cgtccactga agagccactt cgtggtctca agtagcgtgt cctctccaaa tgaaatgaac
      421 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
      481 cagtggagat gtcacatcaa tccacttgct ttgtagacgt ggttggaacc tcttcttttt
      541 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gagagatctt
      601 gaatgatagc ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcta
      661 ctgtcctttc gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgaaa
      721 ttatcctttg ttgaaaagtc tcaatagccc tttgatcttc tgagactgta tctttgacat
      781 ttttggagta gaccagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg
      841 tagacgtggt tggaacctct tctttttcca cgatgctcct cgtggg
//
