LOCUS       sequence-76643-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination Vector for TALEColor-mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NG(T)-mVenus
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(404..406,407..409,410..922)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(449..470)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(710..729)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
       61 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
      121 tcgacggtct cgactcacgc ctgagcaggt agtggctatt gcatccaacg gagggggcag
      181 acccgcactg gagtcaatcg tggcccagct ttcgaggccg gaccccgcgc tggccgcact
      241 cactaatgat catcttgtag cgctggcctg cctcggcgga cgacccgcct tggatgcggt
      301 gaagaagggg ctcccgcacg cgcctgcatt gattaagcgg accaacagaa ggattcccga
      361 gaggacatca catcgagtgg caggcctgca gggaagtgga agtatggtga gcaagggcga
      421 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      481 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      541 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
      601 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      661 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      721 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      781 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      841 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
      901 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
      961 cacccccatc ggcgacggcc ccgtgct
//
