LOCUS       sequence-76644-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Please note- this plasmid is currently not distributed in the MoClo 
            Plant Parts Kit (# 1000000047).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50015
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(155..259)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     327..345
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     regulatory      828..837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
       61 aaaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
      121 cggtcaaggt tctggaccag ttgcgtgagc gcatactctt cctttttcaa tattattgaa
      181 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
      241 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
      301 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtccactga
      361 agagccacgt ggtctcaaag cttacttgta aagctcgtcc attcccatca catcggtaaa
      421 ggccttttgc cactccttga agttgagctc ggtcttggag tgcttgagct cagtcttacg
      481 gaacacgtac ataggctggt tcttaagata gttagcagcc attggcttgg caaaggtgta
      541 ggtggttcta gcagtgcttc tgtatctctt accatttcca gtggtgtaac tccacttaaa
      601 ggtactgatg atggttttgt cgttagggta agttttctta gacctgcacc agtcagcagc
      661 ggtaagagag ttggtcatca caggaccgtc agcagggaaa ccagttccct tcacctgggc
      721 ctctcctttg atgtggcttc cctcgtaggt gtatctgtag ttaacagtaa gggaggcacc
      781 atcttcaaac tgcattgttc tatggacttg gtatccggat ccatcaacca tggcggcctg
      841 gaaaggagac ataccgtcag ggtaaggaag gtactgatgg aatccatatc c
//
