LOCUS       sequence-76645-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL70004
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(56..763)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(901..920)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ataggcgtat cacgaggccc tttcgtccac tgaagagcca cttcgtggtc tcaagcggct
       61 gagcctcgac atgttgtcgc aaaattcgcc ctggacccgc ccaacgattt gtcgtcactg
      121 tcaaggtttg acctgcactt catttggggc ccacatacac caaaaaaatg ctgcataatt
      181 ctcggggcag caagtcggtt acccggccgc cgtgctggac cgggttgaat ggtgcccgta
      241 actttcggta gagcggacgg ccaatactca acttcaagga atctcaccca tgcgcgccgg
      301 cggggaaccg gagttccctt cagtgagcgt tattagttcg ccgctcggtg tgtcgtagat
      361 actagcccct ggggcacttt tgaaatttga ataagattta tgtaatcagt cttttaggtt
      421 tgaccggttc tgccgctttt tttaaaattg gatttgtaat aataaaacgc aattgtttgt
      481 tattgtggcg ctctatcata gatgtcgcta taaacctatt cagcacaata tattgttttc
      541 attttaatat tgtacatata agtagtaggg tacaatcagt aaattgaacg gagaatatta
      601 ttcataaaaa tacgatagta acgggtgata tattcattag aatgaaccga aaccggcggt
      661 aaggatctga gctacacatg ctcaggtttt ttacaacgtg cacaacagaa ttgaaagcaa
      721 atatcatgcg atcataggcg tctcgcatat ctcattaaag caggactcta gcgaacccca
      781 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
      841 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
      901 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gcc
//
