LOCUS       sequence-76650-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL30006
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(160..264)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     332..350
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(431..452)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(692..711)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
       61 ccccaaaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
      121 gcgttcggtc aaggttctgg accagttgcg tgagcgcata ctcttccttt ttcaatatta
      181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
      301 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtcc
      361 actgaagagc cactgtggtc tcaccatggt gagcaagggc gaggagctgt tcaccggggt
      421 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      481 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      541 caagctgccc gtgccctggc ccaccctcgt gaccaccttc agctacggcg tgcagtgctt
      601 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      661 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      721 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      781 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ta
//
