LOCUS       sequence-76670-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL4
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(223..630)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     terminator      926..965
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        967..985
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 taacaaaacg gcatcagcct tcttggaggc ttccagcgcc tcatctggaa gtggaacacc
       61 tgtagcatcg atagcagcac caccaattaa atgattttcg aaatcgaact tgacattgga
      121 acgaacatca gaaatagctt taagaacctt aatggcttcg gctgtgattt cttgaccaac
      181 gtggtcacct ggcaaaacga cgatcttctt aggggcagac attagaatgg tatatccttg
      241 aaatatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga
      301 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta
      361 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg
      421 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg
      481 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc
      541 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc
      601 atcttacgat acctgagtat tcccacagtt aactgcggtc aagatatttc ttgaatcagg
      661 cgccttagac cgctcggcca aacaaccaat tacttgttga gaaatagagt ataattatcc
      721 tataaatata acgtttttga acacacatga acaaggaagt acaggacaat tgattttgaa
      781 gagaatgtgg attttgatgt aattgttggg attccatttt taataaggca ataatattag
      841 gtatgtagat atactagaag ttctcctcga ccggtcgata tgcggtgtga aataccgcac
      901 agatgcgtaa ggagaagcat aaaccgaaac aattaaaggc tccttttgga gccttttttt
      961 ttggacaatt aaccctcact aaagggaaca aaagtctc
//
