LOCUS       sequence-76673-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50008
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     364..380
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     364..380
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     385..404
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        385..403
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    422..521
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(592..613)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
       61 aaaaaacagt cataacaagc catgaaaacc gccaccgcgc cgttaccacc gctgcgttcg
      121 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
      181 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
      241 gcaaccttgg gtagcagcga agtcgaggca tttctgtcct ggctggtcta gaggaggcat
      301 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc
      361 tgccaggaaa cagctatgac catgtaatac gactcactat aggggatatc agctggatgg
      421 caaataatga tttttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
      481 attatttttt ataatgccaa ctttgtacaa gaaagctggg tcgaattcgc ccttcaccgg
      541 tctctttcgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      601 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      661 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      721 cccaccctcg tgaccacctt cagctacggc gtgcagtgct tcagccgcta ccccgaccac
      781 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      841 atcttcttca aggacga
//
