LOCUS       sequence-76678-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH51266
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(146..491)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(183..203)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 taggcgtatc acgaggccct ttcgtccact gaagagccac ttcgtggtct cacattgtat
       61 cgataattgt aaatgtaatt gtaatgttgt ttgttgtttg ttgttgttgg taattgttgt
      121 aaaaatgagc tcttatactc gagcgtgtcc tctccaaatg aaatgaactt ccttatatag
      181 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatgt
      241 cacatcaatc cacttgcttt gtagacgtgg ttggaacctc ttctttttcc acgatgctcc
      301 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga gagatcttga atgatagcct
      361 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttctact gtcctttcga
      421 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgaaatt atcctttgtt
      481 gaaaagtctc aatagccctt tgatcttctg agactgtatc tttgacattt ttggagtaga
      541 ccagagtgtc gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaaa
      601 gactctgtat gaactgttcg ccagtcttga cggcgagttc tgttagatcc tcgatttgaa
      661 tcttagactc catgcatggc cttagattca gtaggaacta cctttttaga gactccaatc
      721 tctattactt gccttggttt atgaagcaag ccttgaatcg tccatactgg aatagtactt
      781 ctgatcttga gaaatatgtc tttctctgtg ttcttgatgc aattagtcct gaatcttttg
      841 actgcatctt taaccttctt gggaaggtat ttgatctcct gga
//
