LOCUS       sequence-76681-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50016
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     intron          371..559
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
ORIGIN
        1 tttcgtccac tgaagagcca cgtggtctca aagcttatca ttcctcacca gcatcagcat
       61 caggggtctt gaaagcatgt tggtactcaa cgattccaag ctcggtgtta gagtgatctt
      121 cctcaactct tctgaaagcg aacataggtc caccgttttg aaggatagaa gggtggatag
      181 cagacttgaa gtgcatgtga gaatccacca cagaagagta gtaaccacca tctctaagtg
      241 agaaggttct ggtgaaagat ccatcgagat cgttatctcc cataggatga agatgctcaa
      301 cagtagcgtt agacctgatg atcttgtcgg tgaagataac agaatcctca gggaatccag
      361 ttcccataac ctgcacatca acaaattttg gtcatatatt agaaaagtta taaattaaaa
      421 tatacacact tataaactac agaaaagcaa ttgctatata ctacattctt ttattttgaa
      481 aaaaatattt gaaatattat attactacta attaatgata attattatat atatatcaaa
      541 ggtagaagca gaaacttacc ttgaaatctc cgatcactct tccagcctcg tatctgtaag
      601 agaagctaac gtgaagaaca ccaccatcct cgtacttctc gatcctagtg ttggtgtatc
      661 caccgttgtt gatagcatga aggaaagggt tctcgtatcc agatgggtaa gttccgaagt
      721 ggtagaatcc gtatcccata acgtgagaaa gaaggtatgg agagaaggta agagcaccct
      781 tggtagactt catcttgtta gtcattcttc cctgctcagg agttccctca ccacctcc
//
