LOCUS       sequence-76684-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of HA-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQHD
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             296..361
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(433..450)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(433..449)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(442..464)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(658..677)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     777..799
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(837..855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaacg
       61 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
      121 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
      181 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
      241 tcgtacaaag tggtgatatc gctagctaga ggtaccgagc tccaattggg atccggacta
      301 caaggaccac gacggcgact ataaggacca cgacatcgac tacaaggatg acgacgacaa
      361 gtgaggatcc ggtgaagtga cccgcaaaaa aaaaggccag gggatcgctc ccccggcctt
      421 ttttttgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      481 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      541 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
      601 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
      661 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
      721 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
      781 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
      841 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agcacccttt
      901 ctcggtcctt caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc
      961 gcgagtccct gctcgaacgc tgcgtccgga ccggct
//
