LOCUS       sequence-76697-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is 
            compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites
            and a T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, Uchino K, Sezutsu 
            H, Tamura T, Zurovec M
  TITLE     Efficient TALEN Construction for Bombyx mori Gene Targeting.
  JOURNAL   PLoS One. 2013 Sep 18;8(9):e73458. doi: 
            10.1371/journal.pone.0073458.
  PUBMED    24058473
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-09-18"; volume: "8(9):e73458. doi"; pages: " 
            10.1371/journal.pone.0073458"
COMMENT     Sequence Label: pBlue-TAL
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..85
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        66..84
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             164..229
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             236..256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccaggcag
       61 tcgactaata cgactcacta tagggcgcgc ctcatttact aaggtgtgct cgaacagtgc
      121 gcattcgcat ctacgtactt gtcacttatt aaaaatcaaa atggattaca aggaccacga
      181 cggcgactac aaggaccacg acatcgacta caaggacgac gacgacaaga tggctcccaa
      241 gaagaagcgt aaagtgggca tccacggagt ccctatggtt gacttgcgca ctctgggcta
      301 ctcccagcaa cagcaagaga agatcaagcc caaggtgcgc tctaccgtcg ctcagcacca
      361 cgaagccctg gttggtcacg gcttcactca cgctcacatc gtggctctga gccagcaccc
      421 agctgctctc ggaacagtgg ctgtcaagta ccaagatatg atcgctgcct tgccggaggc
      481 tacccacgaa gccatcgttg gagtgggcaa gcagtggtcc ggtgctcgtg ctctggaggc
      541 tctgctgact gtggctggtg aactcagagg accacctttg caactggata caggacagtt
      601 gctgaagatc gccaagaggg gaggagtgac cgccgttgaa gccgtgcatg cttggcgtaa
      661 cgccctgacc ggtgctcccc tggagacggg cgccgctaca gggcgcgtcc cattcgccat
//
