LOCUS       sequence-76702-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for TALEN with TALEColor Backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NI(A)-Fok1
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             409..996
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
       61 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
      121 gcaggtcgac ggtctcgact cacgcctgag caggtagtgg ctattgcatc caacatcggg
      181 ggcagacccg cactggagtc aatcgtggcc cagctttcga ggccggaccc cgcgctggcc
      241 gcactcacta atgatcatct tgtagcgctg gcctgcctcg gcggacgacc cgccttggat
      301 gcggtgaaga aggggctccc gcacgcgcct gcattgatta agcggaccaa cagaaggatt
      361 cccgagagga catcacatcg agtggcaggc ctgcagggaa gtggttccca actcgtgaag
      421 agtgaacttg aggagaaaaa gtcggagctg cggcacaaat tgaaatacgt accgcatgaa
      481 tacatcgaac ttatcgaaat tgctaggaac tcgactcaag acagaatcct tgagatgaag
      541 gtaatggagt tctttatgaa ggtttatgga taccgaggga agcatctcgg tggatcacga
      601 aaacccgacg gagcaatcta tacggtgggg agcccgattg attacggagt gatcgtcgac
      661 acgaaagcct acagcggtgg gtacaatctt cccatcgggc aggcagatga gatgcaacgt
      721 tatgtcgaag aaaatcagac caggaacaaa cacatcaatc caaatgagtg gtggaaagtg
      781 tatccttcat cagtgaccga gtttaagttt ttgtttgtct ctgggcattt caaaggcaac
      841 tataaggccc agctcacacg gttgaatcac attacgaact gcaatggtgc ggttttgtcc
      901 gtagaggaac tgctcattgg tggagaaatg atcaaagcgg gaactctgac actggaagaa
      961 gtcagacgca agtttaacaa tggcgagatc aatttccg
//
